Guidelines for the Use of Antiretroviral Agents in Pediatric HIV Infection
Management of Treatment-Experienced Infants, Children, and Adolescents
Choice of Next Antiretroviral Regimen for Virologic Treatment Failure with Evidence of Drug Resistance
(Last updated:11/1/2012; last reviewed:11/1/2012)
- Antiretroviral (ARV) regimens should be chosen based on treatment history and drug-resistance testing, including both past and current resistance test results (AI*).
- The new regimen should include at least two, but preferably three, fully active ARV medications with assessment of anticipated ARV activity based on past treatment history and resistance test results (AII*).
- Interpretation of resistance test results showing complex combinations of mutations and assessment of future treatment options should be made in collaboration with a pediatric HIV specialist (AI*).
- Use of novel agents with limited available pharmacokinetic and/or safety data in pediatric populations should be undertaken only in collaboration with a pediatric HIV specialist (AIII).
|Rating of Recommendations: A = Strong; B = Moderate; C = Optional
Rating of Evidence: I = One or more randomized trials in children† with clinical outcomes and/or validated endpoints; I* = One or more randomized trials in adults with clinical outcomes and/or validated laboratory endpoints with accompanying data in children† from one or more well-designed, nonrandomized trials or observational cohort studies with long-term clinical outcomes; II = One or more well-designed, nonrandomized trials or observational cohort studies in children† with long-term outcomes; II* = One or more well-designed, nonrandomized trials or observational studies in adults with long-term clinical outcomes with accompanying data in children† from one or more similar nonrandomized trials or cohort studies with clinical outcome data; III = expert opinion
† Studies that include children or children and adolescents but not studies limited to postpubertal adolescents
After reaching a decision that a change in therapy is needed, a clinician should attempt to identify at least two, but preferably three, fully active antiretroviral (ARV) agents from at least two different classes on the basis of resistance test results, prior ARV exposure, acceptability to the patient, and likelihood of adherence.1-5 This often requires using agents from one or more drug classes that are new to the patient. Substitution or addition of a single drug to a failing regimen should not be done because it is unlikely to lead to durable virologic suppression and will likely result in additional drug resistance. A drug may be new to the patient but have diminished antiviral potency because of the presence of drug-resistance mutations that confer cross resistance within a drug class. In children who are changing therapy owing to the occurrence or progression of abnormal neurodevelopment, the new treatment regimen should include agents (such as zidovudine) that are known to achieve higher concentrations in the central nervous system.6-10
A change to a new regimen must include an extensive discussion of treatment adherence and potential toxicity with a patient in an age- and development-appropriate manner and with a patient’s caregivers. Clinicians must recognize that conflicting requirements of some medications with respect to food and concomitant medication restrictions may complicate administration of a regimen. Timing of medication administration is particularly important to ensure adequate ARV drug exposures throughout the day. Palatability, size and number of pills, and dosing frequency all need to be considered when choosing a new regimen.11
Choice of Therapy with Viral Resistance to Current Therapy: Goal of Complete Virologic Suppression
Determination of a new regimen with the best chance for complete virologic suppression in children who have already experienced treatment failure should be made in collaboration with a pediatric HIV specialist. ARV regimens should be chosen based on treatment history and drug-resistance testing to optimize ARV drug potency in the new regimen. A general strategy for regimen change is shown in Table 20, although as additional agents are licensed and studied for use in children, newer strategies that are better tailored to the needs of each patient may be constructed.
If a child has received initial therapy with a non-nucleoside reverse transcriptase inhibitor (NNRTI)-based regimen, a change to a protease inhibitor (PI)-based regimen is recommended. Resistance to the NNRTI nevirapine results in cross-resistance to the NNRTI efavirenz, and vice versa. However, the newer NNRTI etravirine retains activity against nevirapine- or efavirenz-resistant virus (see below). If a child received initial therapy with a PI-based regimen, a change to an NNRTI-based regimen is generally recommended. Lopinavir/ritonavir-based regimens have also been shown to have durable ARV activity in some PI-experienced children.12-14 Choice of the new dual-nucleoside reverse transcriptase inhibitor (NRTI) component is particularly important when constructing a regimen because the choice of an insufficiently potent NRTI may result in selection of additional NRTI-related drug-resistance mutations. Resistance testing is essential to properly select a potent NRTI combination, and interpretation of these results should take place in collaboration with an expert in pediatric HIV infection (see Antiretroviral Drug-Resistance Testing).
The availability of new drugs in existing classes (e.g., the NNRTI etravirine) and new classes of drugs (e.g., integrase inhibitors) increases the likelihood of finding three active drugs, even for children with extensive drug resistance (Table 20). In studies of adults, etravirine retains activity against nevirapine- or efavirenz-resistant viruses when used in a regimen that also contains darunavir/ritonavir and if the number of NNRTI resistance-associated mutations is limited.15,16 Etravirine in combination with ritonavir-boosted darunavir, as part of a new combination antiretroviral therapy (cART) regimen, has been shown to be a safe and effective option for children in whom cART fails.17,18 Etravirine is approved for use in children aged ≥6 years;17,19 studies in younger children are under way. Studies of treatment-experienced adult and adolescent patients have shown that using one or more new class(es) of drug (e.g., integrase inhibitors, entry inhibitors), often coupled with a ritonavir-boosted PI (e.g., darunavir) in PI-experienced patients with multidrug-resistant virus, is associated with good virologic responses.20-23 Raltegravir, in combination with optimized background therapy, was safe and effective in treatment-experienced children aged 2 to 16 years,24 for whom it is Food and Drug Administration (FDA)-approved. Use of newer agents in novel combinations is becoming more common in aging perinatally infected youth in the United States.25 It is important to review individual drug profiles for information about drug interactions and dose adjustment when devising a regimen for children with multi-class drug resistance. Appendix A: Pediatric Antiretroviral Drug Information provides more detailed information on drug formulation, pediatric and adult dosing, and toxicity, as well as discussion of available pediatric data for the approved ARV drugs, including new drugs in existing classes such as darunavir and agents in new classes of drugs such as CCR5 antagonists (e.g., maraviroc, approved for use in adolescents aged ≥16 years) and integrase inhibitors (e.g., raltegravir, approved for use in children aged ≥2 years [FDA, December 21, 2011]).
Previously prescribed drugs that were discontinued because of poor tolerance or poor adherence may sometimes be reintroduced if ARV resistance did not develop and if prior difficulties with tolerance and adherence can be overcome (such as by switching from a liquid to pill formulation or to a new formulation [such as ritonavir tablet]). Limited data in adults suggest that continuation of lamivudine can contribute to suppression of HIV replication despite the presence of lamivudine resistance mutations and can maintain lamivudine mutations (184V) that can partially reverse the effect of other mutations conferring resistance to zidovudine, stavudine, and tenofovir.26-28 The use of new drugs that have been evaluated in adults but have not been fully evaluated in children may be justified and ideally is done in the framework of a clinical trial (see The Use of Antiretroviral Agents Not Approved for Use in Children). Expanded access programs or clinical trials may be available. New drugs should be used in combination with at least one, and ideally two, additional active agents.
The HIV entry inhibitor enfuvirtide is approved for use in heavily treatment-experienced patients based on potent ARV activity in heavily treatment-experienced adults; it has been approved for use in children aged ≥6 years.29,30 Studies have helped establish safety, appropriate dosing, and efficacy of enfuvirtide in treatment-experienced children aged ≥6 years.31,32 Enfuvirtide must be administered by subcutaneous injection twice daily, a disadvantage that presents a greater challenge to adherence in adolescents than in younger children. Enfuvirtide can be considered an option when designing a new regimen for children in whom multiple classes of ARV medications have failed, but newer and better tolerated agents have largely supplanted use of enfuvirtide.
Pharmacokinetic (PK) studies of certain dual-boosted PI regimens (lopinavir/ritonavir with saquinavir and lopinavir/ritonavir with atazanavir/ritonavir) suggest that PK targets for both PIs can be achieved or exceeded when used in combination in adults33-35 and in children.36-38 PK studies of other dual-boosted PI combinations are limited but suggest inadequate drug levels of one or both PIs.39,40 A study in Thailand of 50 PI-naive but NRTI +/- NNRTI-experienced children treated with a combination of lopinavir/ritonavir (230/57.5 mg/m2 twice daily) and saquinavir (50 mg/kg twice daily, maximum dose 1000 mg) demonstrated trough levels of both PIs at or above therapeutic targets and complete viral suppression at 48 weeks in ≥50% of patients. The use of multidrug regimens, sometimes including up to 3 PIs and/or 2 NNRTIs, has shown efficacy in a pediatric case series;41 however, multidrug regimens should be used cautiously because of their complexity, poor tolerability, and unfavorable drug-drug interactions. Therapeutic drug monitoring may be helpful for confirming therapeutic PI levels when using PIs in combinations that result in complex drug interactions or when there is partially reduced PI activity because of the presence of drug-resistance mutations (see Role of Therapeutic Drug Monitoring in Management of Treatment Failure). Availability of newer potent PIs and new classes of ARV drugs (integrase and CCR5 inhibitors) may lessen the need for dual-PI regimens.
When searching for at least two fully active agents in cases of extensive drug resistance, clinicians should consider the potential availability and future use of newer therapeutic agents that may not be studied or approved in children or may be in clinical development (see The Use of Antiretroviral Agents Not Approved for Use in Children). Information concerning potential clinical trials can be found at /clinical_trials and through collaboration with a pediatric HIV specialist. Children should be enrolled in clinical trials of new drugs whenever possible.
Therapeutic Options When Two Fully Active Agents Cannot Be Identified or Administered
It may be impossible to provide an effective and sustainable therapeutic regimen because no combination of currently available agents is active against extensive drug-resistant virus in a patient or because a patient is unable to adhere to or tolerate cART.
In such cases, non-suppressive regimens (or holding regimens) are sometimes used pending availability of additional active, tolerable drugs or improvement in ability to adhere. This interim strategy allows for the overall objective of preventing clinical and immunological deterioration until new agents are available to design a regimen that can be expected to achieve undetectable plasma viral load. This approach should be regarded as acceptable but not ideal. Such patients should be followed more closely than those with stable virologic status, and the potential to successfully initiate a fully suppressive cART regimen should be reassessed at every opportunity.
Even when NRTI drug-resistance mutations are present, patients can derive immunologic and clinical benefit despite persistent viremia from treatment with lamivudine monotherapy or with lamivudine or emtricitabine in combination with one or more other NRTIs, such as zidovudine, stavudine, abacavir, or tenofovir.42,43
The newer NNRTI etravirine retains activity against many nevirapine- or efavirenz-resistant viruses with a limited number of NNRTI resistance-associated mutations. Ongoing use of efavirenz or nevirapine as part of a failing regimen should be avoided because it may lead to accumulation of additional NNRTI resistance mutations that will reduce etravirine activity and preclude its use in a future, suppressive regimen,44 and it may allow for accumulation of additional NRTI resistance.45
Continued use of a PI in the face of persistent viremia can lead to accumulation of additional mutations conferring resistance to that PI as well as other, newer PIs. Such acquisition of additional PI drug resistance occurs slowly, especially if the viral load is relatively low.46-49 However, continued PI use in the presence of resistance may limit viral replication and be beneficial to some patients.
When clinical or immunologic deterioration occurs while patients are receiving such holding regimens, it is important to re-assess patient readiness and regimen availability. It may be appropriate to use investigational agents or agents approved for older age groups as second fully active drugs in the new regimen (see The Use of Antiretroviral Agents Not Approved for Use in Children). In general, a single, new, fully active agent should not be added to non-suppressive holding regimens because resistance is likely to develop quickly.
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Table 20. Options for Regimens with at Least Two Fully Active Agents with Goal of Virologic Suppression in Patients With Failed Antiretroviral Therapy and Evidence of Viral Resistancea
||Recommended Change (in order of relative preference)a
|2 NRTIs + NNRTI
- 2 NRTIs + PI
- 2 NRTI + integrase inhibitorb
|2 NRTIs + PI
- 2 NRTIs + NNRTI
- 2 NRTIs + alternative RTV-boosted PI
- 2 NRTIs + integrase inhibitorb
- NRTI(s) + integrase inhibitor + (NNRTI or alternative RTV-boosted PI)
- 2 NRTIs + (NNRTI or PI)
- 2 NRTIs + integrase inhibitorb
- Integrase inhibitorb + 2 other active agents (chosen from NNRTI, PI, NRTI[s])
|Failed regimen(s) that included NRTI(s), NNRTI(s), and PI(s)
- > 1 NRTI + RTV-boosted PI
- NRTI(s) + RTV-boosted PI + integrase inhibitorb (consider adding T-20 and/or MVC,c if additional active drug[s] needed)
- NRTI(s) + RTV-boosted DRV, LPV or SQV + ETR (consider adding one or more of MVC,c T-20, or integrase inhibitor,b if additional active drug[s] needed)
- > 1 NRTI + 2 RTV-boosted PIs (LPV/r + SQV, LPV/r + ATV) (consider adding T-20 or an integrase inhibitorb if additional active drug[s] needed)
- Katlama C, Haubrich R, Lalezari J, et al. Efficacy and safety of etravirine in treatment-experienced, HIV-1 patients: Pooled 48 week analysis of two randomized, controlled trials. AIDS. Nov 13 2009;23(17):2289-2300. Available at http://www.ncbi.nlm.nih.gov/pubmed/19710593.
- Steigbigel RT, Cooper DA, Teppler H, et al. Long-term efficacy and safety of Raltegravir combined with optimized background therapy in treatment-experienced patients with drug-resistant HIV infection: Week 96 results of the BENCHMRK 1 and 2 Phase III trials. Clin Infect Dis. Feb 15 2010;50(4):605-612. Available at http://www.ncbi.nlm.nih.gov/pubmed/20085491.
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- Antinori A, Giancola ML, Grisetti S, et al. Factors influencing virological response to antiretroviral drugs in cerebrospinal fluid of advanced HIV-1-infected patients. AIDS. Sep 27 2002;16(14):1867-1876. Available at http://www.ncbi.nlm.nih.gov/pubmed/12351946.
- Antinori A, Perno CF, Giancola ML, et al. Efficacy of cerebrospinal fluid (CSF)-penetrating antiretroviral drugs against HIV in the neurological compartment: Different patterns of phenotypic resistance in CSF and plasma. Clin Infect Dis. Dec 15 2005;41(12):1787-1793. Available at http://www.ncbi.nlm.nih.gov/pubmed/16288405.
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- Letendre S, Marquie-Beck J, Capparelli E, et al. Validation of the CNS Penetration-Effectiveness rank for quantifying antiretroviral penetration into the central nervous system. Arch Neurol. Jan 2008;65(1):65-70. Available at http://www.ncbi.nlm.nih.gov/pubmed/18195140.
- Patel K, Ming X, Williams PL, et al. Impact of HAART and CNS-penetrating antiretroviral regimens on HIV encephalopathy among perinatally infected children and adolescents. AIDS. Sep 10 2009;23(14):1893-1901. Available at http://www.ncbi.nlm.nih.gov/pubmed/19644348.
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- Galan I, Jimenez JL, Gonzalez-Rivera M, et al. Virological phenotype switches under salvage therapy with lopinavir-ritonavir in heavily pretreated HIV-1 vertically infected children. AIDS. Jan 23 2004;18(2):247-255. Available at http://www.ncbi.nlm.nih.gov/pubmed/15075542.
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- Vingerhoets J, Peeters M, Azijn H, et al. An update of the list of NNRTI mutations associated with decreased virological response to etravirine: Multivariate analyses on the pooled DUET-1 and DUET-2 clinical trial data. Abstract 24. Antivir Ther. 2008;13(Suppl 3):A26.
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