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Clinical Trials

MainTitle

MK-0518 Intensification And HDAC Inhibition In Depletion Of Resting CD4+ T Cell HIV Infection

This study has been terminated
( Due to insufficient funds )

Sponsor
University of North Carolina, Chapel Hill

Collaborator
National Institute of Allergy and Infectious Diseases (NIAID)
Abbott
Merck Sharp & Dohme Corp.

Information provided by (Responsible Party)
David Margolis, MD, University of North Carolina, Chapel Hill

ClinicalTrials.gov Identifier
NCT00614458

First received: January 31, 2008
Last updated: October 24, 2011
Last Verified: September 2011
History of Changes
Purpose

Purpose

The purpose of this research study is to see if HIV that persists despite current antiviral therapy can be targeted by new treatments. We will see if adding Raltegravir (MK-0518) and Valproic acid (VPA) to current ART can decrease the amount of latent HIV.

Condition Intervention Phase
HIV Infections

Drug : Raltegravir; valproic acid
Phase 2

Study Type: Interventional
Study Design: Allocation: Non-Randomized
Intervention Model: Single Group Assignment
Masking: None (Open Label)
Primary Purpose: Treatment
Official Title: 10493 - MK-0518 Intensification and HDAC Inhibition in Depletion of Resting CD4+ T Cell HIV Infection

Further study details as provided by David Margolis, MD, University of North Carolina, Chapel Hill:

Primary Outcome Measures

  • A Change in the Number of HIV Infected Cells. [ Time Frame: 20 weeks ]
    A change in infected cells from prior to the initiation of VPA and MK0518 to after 20 weeks of treatment.

Enrollment: 6
Study Start Date: April 2007
Study Completion Date: September 2008
Estimated Primary Completion Date: September 2008 (Final data collection date for primary outcome measure)

Arms Assigned Interventions
Experimental: Raltegravir and VPA to ART
raltegravir 400mg po BID; valproic acid 1000mg - 2000mg daily
Drug: Raltegravir; valproic acid

raltegravir 400mg po BID; valproic acid 1000mg - 2000mg daily

Other Name:
  • raltegravir (MK 0518)
  • Valproic acid (VPA) (depakote)

Detailed Description:

The purpose of this research study is to see if HIV that persists despite current antiviral therapy can be targeted by new treatments. Eradicating this hidden, persistent virus (which we will call "latent HIV") may some day allow HIV to be eliminated from an infected person. Recently it has been discovered that a class of medicines, called histone deacetylase (HDAC) inhibitors, may cause the HIV that hides within some of the cells of your body to be expressed ("unmasked"). The HDAC inhibitor we will use in this study to unmask or flush out the latent HIV within your cells is called valproic acid (VPA) and is commonly used to treat seizures and depression under the brand name Depakote. VPA has been safely given to people with HIV for the treatment of seizures or depression.
In our first study, we recruited 4 volunteers with HIV-1 infection taking antiviral therapy in whom there were <50 copies/ml (undetectable) of HIV virus RNA in their blood for over 6 months. In addition to continuing their anti-HIV medications, we started them on Fuzeon, a new injected anti-HIV medication, for one month. We then added VPA to the anti-HIV medications and Fuzeon for 3 months. At the end of the study, we found that latent HIV was significantly decreased. No safety problems with VPA and antiviral medicines were noted. However, having initiated Fuzeon and VPA simultaneously we could not determine with confidence that our findings were due to the effect of VPA alone.
In the next study we added VPA to the therapy of patients taking standard antiretroviral therapy (ART). Eight patients have been studied so far, but only three have yet had a significant decrease in latent HIV. It may be that VPA does not work in everyone, or that stronger HIV therapy is needed in some people to drain latent HIV. And in a different study, no decrease at all was found in the number of infected cells in people taking ART who were prescribed valproate for other reasons.
In this study, we wish to add a different new anti-HIV medicine, which is a pill, Raltegravir (MK-0518). Raltegravir blocks the virus from permanently entering the DNA of your T cells, and so is called an integrase inhibitor. Raltegravir is investigational. This means the U.S. Food and Drug Administration (FDA) have not yet approved it for sale. We will see if adding Raltegravir and VPA to your current ART can decrease the amount of latent HIV. If adding Raltegravir and VPA to your ART has no effect, you will have reached the end of the study. If adding Raltegravir and VPA decreases latent infection, we will stop VPA but continue Raltegravir to see if this new integrase inhibitor decreases latent infection. This study does not expect to cure your HIV, but only to take the first step towards that far-away goal.
You are asked to join this study because you are infected with the HIV-1 virus; you are 18 years of age; you are able and willing to sign an informed consent.; you are able to have laboratory tests within the study specific criteria; and you have adequate vein access for leukopheresis.

Eligibility

Eligibility

Ages Eligible for Study: 18 Years and older  
Sexes Eligible for Study: All  
Accepts Healthy Volunteers: No  

Criteria

Inclusion Criteria:

  • HIV-1 infection
  • Men and women age ≥18 years.
  • Ability and willingness of subject or legal guardian/representative to give written informed consent.
  • Karnofsky performance status >70.
  • Willing to adhere to protocol therapy and judged adherent to antiretroviral therapy, and can comply with time requirements for protocol-specified visits and evaluations.
  • On potent antiretroviral therapy, defined as at least 2 nucleoside reverse transcriptase inhibitors (NRTIs) plus at least 1 protease inhibitor (PI) or non-nucleoside reverse transcriptase inhibitor (NNRTI), without changes in the 24 weeks immediately prior to entry. Prior changes in or elimination of medications for easier dosing schedule, intolerance, or toxicity are permitted.
  • Have no contraindications to valproic acid (VPA) therapy (pregnancy, bleeding disorders, history of pancreatitis, history of hepatitis).
  • Have adequate vascular access for leukopheresis
  • Plasma HIV-1 RNA never > 50 copies/ml on two consecutive occasions for ≥ 6 months.
  • CD4 cell count > 300 cells/µl.
  • Screening serum fasting glucose below 120 mg/dl, creatinine no more than 1.5 times the upper limit of the normal range, serum AST, ALT, and total bilirubin less than 2 times the upper limit of the normal range, and fasting triglycerides < 400mg/dl.
  • Female study volunteers who are not of reproductive potential or whose male partner(s) has undergone successful vasectomy with documented azoospermia or has documented azoospermia for any other reason, are eligible without requiring the use of contraception.
  • All women of reproductive potential must have a negative urine beta-HCG pregnancy test performed at screening, day 0, week 4, week 8, week 12, and every scheduled study visit thereafter.
  • All study volunteers must agree not to participate in a conception process and, if participating in sexual activity that could lead to pregnancy, the female study volunteer/male partner must use two reliable methods of contraception simultaneously while receiving the protocol-specified medications and for 6 weeks after stopping the medications. Subjects must use a reliable barrier method of contraception along with another form of contraception. For women receiving ritonavir, estrogen-based contraceptives are not reliable and an alternative method should be used.


Exclusion Criteria:
  • Receiving zidovudine (AZT). Due to the inhibition of zidovudine metabolism mediated by Valproic acid (VPA) a theoretical increase risk of zidovudine-induced anemia exists.
  • Using drugs known to interact with valproate or raltegravir (MK-0518)
  • Pregnant or nursing.
  • Anemic (Hemoglobin < 10gm/dl)
  • Seropositive for hepatitis C RNA or hepatitis B surface antigen within 90 days prior to entry.
  • Have signs or symptoms of hepatic decompensation
  • Received blood transfusions or hematopoetic growth factors within 90 days.
  • Use of any of the following within 90 days prior to entry: systemic cytotoxic chemotherapy, investigational agents, immunomodulators.
  • Active drug or alcohol use or dependence.
  • Serious illness requiring systemic treatment or hospitalization until subject either completes therapy or has been clinically stable on therapy, in the opinion of the site investigator, for at least 90 days prior to entry.
  • Compulsorily detained (involuntarily incarcerated) for treatment of either a psychiatric illness or a physical illness, e.g., infectious disease.
  • Treatment for an active AIDS-defining opportunistic infection within 90 days prior to
screening.
For this pilot study of no direct benefit to subjects, non-English speaking patients are excluded. In the future, if this study is expanded to include a larger sample size, this exclusion criterion may be removed.

contacts and locations

Contacts and Locations

Choosing to participate in a study is an important personal decision.Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the Contacts provided below.For general information, see Learn About Clinical Studies.

Please refer to this study by its ClinicalTrials.gov identifier: NCT00614458

Locations

United States, North Carolina
University of North Carolina at Chapel Hill
Chapel Hill, North Carolina, United States, 27599

Sponsors and Collaborators

University of North Carolina, Chapel Hill
National Institute of Allergy and Infectious Diseases (NIAID)
Abbott
Merck Sharp & Dohme Corp.

Investigators

Principal Investigator: David M Margolis, MD University of North Carolina, Chapel Hill
More Information

More Information


Responsible Party: David Margolis, MD, Principal Investigator, University of North Carolina, Chapel Hill  
ClinicalTrials.gov Identifier: NCT00614458   History of Changes  
Other Study ID Numbers: 10493  
  R01AI064074  
  P30AI050410  
  U01AI067854  
Study First Received: January 31, 2008  
Last Updated: October 24, 2011  

Keywords provided by David Margolis, MD, University of North Carolina, Chapel Hill:

latent HIV
resting CD4+ T cell
valproic acid (VPA)
Raltegravir (MK-0518) intensification
HIV replication
treatment experienced

Additional relevant MeSH terms:
Infection
Communicable Diseases
HIV Infections
Acquired Immunodeficiency Syndrome
Raltegravir Potassium
Valproic Acid

ClinicalTrials.gov processed this data on October 16, 2017
This information is provided by ClinicalTrials.gov.