Clinical Trials

MainTitle

A Phase I Study of T-Cells Genetically Modified at the CCR5 Gene by Zinc Finger Nucleases SB-728mR in HIV-Infected Patients

This study is ongoing, but not recruiting participants.
Sponsor
University of Pennsylvania

Collaborator
National Institute of Allergy and Infectious Diseases (NIAID)

Information provided by (Responsible Party)
University of Pennsylvania
ClinicalTrials.gov Identifier
NCT02388594

First received: February 24, 2015
Last updated: August 21, 2017
Last Verified: August 2017
History of Changes
Purpose

Purpose

This is a triple cohort, open-label pilot study of the safety and antiviral activity of a single infusion of autologous CD4+ T cells genetically modified at the CCR5 gene by Zinc Finger Nucleases SB-728mR (ZFN Modified CD4+ T Cells) using electroporated mRNA with or without the prior administration of two different doses of cyclophosphamide.

Condition Intervention Phase
Human Immunodeficiency Virus (HIV)

Drug : ZFN Modified CD4+ T Cells
Drug : Cyclophosphamide
Phase 1

Study Type: Interventional
Study Design: Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)
Primary Purpose: Treatment
Official Title: A Phase I Study of T-Cells Genetically Modified at the CCR5 Gene by Zinc Finger Nucleases SB-728mR in HIV-Infected Patients, With or Without the CCR5 Delta-32 Mutation, Pre-treated With Cyclophosphamide

Further study details as provided by University of Pennsylvania:

Primary Outcome Measures

  • Number of participants with adverse events [ Time Frame: 6 months post treatment ]

Estimated Enrollment: 15
Study Start Date: April 2015
Estimated Study Completion Date: January 2019
Estimated Primary Completion Date: October 2018 (Final data collection date for primary outcome measure)

Arms Assigned Interventions
Experimental: ZFN Modified CD4+ T Cell
ZFN Modified CD4+ T Cell
Drug: ZFN Modified CD4+ T Cells
Other Name: SB-728mR
Experimental: ZFN Modified CD4+ T Cell with Cyclophosphamide
ZFN Modified CD4+ T Cell with Cyclophosphamide
Drug: ZFN Modified CD4+ T Cells
Other Name: SB-728mR
Drug: Cyclophosphamide
Eligibility

Eligibility

Ages Eligible for Study: 18 Years and older  
Sexes Eligible for Study: All  
Accepts Healthy Volunteers: No  

Criteria

Inclusion Criteria:

  • HIV-1 infection, as documented by a rapid HIV test or any FDA-approved HIV-1 Enzyme or Chemiluminescence Immunoassay (E/CIA) test kit and confirmed by Western blot at any time prior to study entry or HIV antigen, plasma HIV-1 RNA, or second antibody test by a method other than rapid HIV and E/CIA. Alternatively, if a rapid HIV test or any FDA-approved HIV-1 Enzyme or Chemiluminescence Immunoassay (E/CIA) test kit is not available, two HIV-1 RNA values ≥ 2000 copies/mL at least 24 hours apart performed by any laboratory that has CLIA certification, or its equivalent, may be used to document infection.
  • CD4+ T cell count of ≥450 cells/mm3 at screen; and a documented CD4 nadir of not lower than 200 cells/mm3.
  • Adequate venous access and no other contraindications for leukapheresis.
  • Laboratory values obtained at screen:
    1. Hemoglobin: ≥ 10.0 (males); ≥ 9.5 (females) g/dL
    2. Absolute neutrophil count (ANC): ≥ 1000/mm3
    3. Platelet count: ≥ 100,000/mm3
    4. Aspartate aminotransferase (AST) or alanine aminotransferase (ALT): ≤ 2.5 times the upper limit of normal (ULN).
  • Subjects must be willing to comply with study-mandated evaluations; including not changing their antiretroviral regimen (unless medically indicated) for 2 months in step 2 or until undergoing the analytical treatment interruption.
  • Be male or female, 18 years of age and older.
  • Ability and willingness of subject to provide informed consent.
  • Have a Karnofsky Performance Score of 70 or higher.
  • Have no polymorphisms in the CCR5 ZFN target region as determined by Cel I snp assay at screening.
  • Subjects in Cohorts 2 and 3: LVEF > or equal to 40%
  • Clinically stable on their first or second HAART regimen. Changes while the patient HIV viral load is undetectable does not count toward the number of ART regimens used, only changes made for virologic failure (for example an individual switching from an NNRTI-based regimen to an integrase inhibitor based regimen while the HIV viral load is undetectable will still be in their first regimen). Site investigator anticipates that a fully active alternative ART regimen could be constructed in the event of virologic failure on the current ART regimen. The current regimen should have no changes within 4 weeks of enrollment. Subjects must be willing to continue on current antiretroviral therapy for the duration of the study except for the duration of the 16 week analytical treatment interruption. NOTE: Subject's ART regimen must be in accordance with the Department of Health and Human Services Document "Guidelines for the Use of Antiretroviral Agents in HIV-1 Infected Adults and Adolescents."
  • HIV-1 RNA undetectable by ultrasensitive assay copies/ml obtained at study screening visit or within 60 days prior to study screening visit performed with an ultrasensitive HIV-1 PCR assay. All subjects must have received at least 18 months of therapy and have HIV-1 RNA <50 copies/mL using a FDA-approved assay for at least 48 weeks prior to enrollment. HIV-1 RNA must be measured at least once in the 24 weeks prior to enrollment and at least 3 days before the screening measure. Single determinations that are between ≥50 and <500copies/mL (i.e., blips) are allowed as long as the preceding and subsequent determinations are <50 copies/mL. The screening value may serve as the subsequent determination <50 copies/mL following a blip. NOTE: subjects who have participated in other trials using ATI's will be permitted since detectable virus during the interruption does not represent virologic failure. These subjects should have at least 24 weeks of VL <50 copies/mL.
  • Have a recorded viral load set point

  • Exclusion Criteria:
  • Acute or chronic hepatitis B or hepatitis C infection (as further defined in section ~#o1~3.4 and 6.3.8 of this protocol)
    • Current or prior AIDS diagnosis.
    • History of cancer or malignancy, with the exception of successfully treated basal cell or squamous cell carcinoma of the skin
    • History or any features on physical examination indicative of active or unstable cardiac disease or hemodynamic instability. NOTE: subjects with a history of cardiac disease may participate with a physician's approval.
    • History or any features on physical examination indicative of a bleeding diathesis.
    • Have been previously treated with any HIV experimental vaccine within 6 months prior to screening, or any previous gene therapy using an integrating vector. Note: Subjects treated with placebo in an HIV vaccine study will not be excluded if documentation that they received placebo is provided.
    • Use of chronic systemic corticosteroids, hydroxyurea, or immunomodulating agents (e.g., interleukin-2, interferon-alpha or gamma, granulocyte colony stimulating factors, etc.) within 30 days prior to study screening visit. NOTE: Recent or current use of inhaled steroids is not exclusionary. If subjects are prescribed a brief course of oral corticosteroids, the use should be limited to less than 7 days. Use of steroids before apheresis and immune assessment blood draws should be discouraged as it will affect white blood cell function.
    • Breast-feeding, pregnant, or unwilling to use acceptable methods of birth control.
    • Anticipated use of aspirin, dyprydamole, warfarin or any other medication that is likely to affect platelet function or other aspects of blood coagulation during the 2-week period prior to leukapheresis.
    • Active drug or alcohol use or dependence that, in the opinion of the site investigator, would interfere with adherence to study requirements.
    • Serious illness requiring systemic treatment and/or hospitalization within 30 days prior to study screening visit.
    • Asymptomatic baseline serum chemistry elevations in LFTs, bilirubin, lipase and serum creatinine due to HAART medication are not exclusionary, when in the opinion of the investigator, the abnormalities are not attributable to intrinsic hepatorenal disease. Such baseline elevations must be due to HAART.
    • Receipt of vaccination within 30 days prior to study screening visit. NOTE: It is recommended that subjects enrolling into this study should have completed their routine vaccinations (hepatitis A, hepatitis B, pneumococcus, and tetanus diphtheria booster) at least 30 days prior to screening for the study.
    • Have an allergy or hypersensitivity to study product excipients (human serum albumin,
    DMSO and Dextran 40).

    contacts and locations

    Contacts and Locations

    Choosing to participate in a study is an important personal decision.Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the Contacts provided below.For general information, see Learn About Clinical Studies.

    Please refer to this study by its ClinicalTrials.gov identifier: NCT02388594

    Locations

    United States, Pennsylvania
    University of Pennsylvania
    Philadelphia, Pennsylvania, United States, 19104

    Sponsors and Collaborators

    University of Pennsylvania
    National Institute of Allergy and Infectious Diseases (NIAID)
    More Information

    More Information


    Responsible Party: University of Pennsylvania  
    ClinicalTrials.gov Identifier: NCT02388594   History of Changes  
    Other Study ID Numbers: SB-728mR  
      U01AI104400  
    Study First Received: February 24, 2015  
    Last Updated: August 21, 2017  

    Additional relevant MeSH terms:
    Immunologic Deficiency Syndromes
    Acquired Immunodeficiency Syndrome
    HIV Infections
    Cyclophosphamide

    ClinicalTrials.gov processed this data on December 15, 2017
    This information is provided by ClinicalTrials.gov.