Monomeric DAPTAOther Names: Adaptavir, D-Ala1-peptide T-amide, DAPTA, Peptide T, RAP101, mDAPTA, monomeric D-Ala-peptide T-amide Drug Class: Entry Inhibitor
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(Compound details obtained from ChemIDplus Advanced,1 NIAID Therapeutics Database,2 and RAPID Pharmaceuticals press release3)
Mechanism of Action: HIV-1 entry inhibitor. Monomeric DAPTA (mDAPTA) is a reformulated monomeric form of peptide T ([D-Ala1] Peptide T Amide; DAPTA), a synthetic octapeptide compound derived from the gp120 V-2 region of HIV. Peptide T is a selective CCR5 co-receptor antagonist that prevents viral entry by directly binding to the CCR5 co-receptor and subsequently inhibiting the interaction between HIV-1 gp120 and CCR5.4-7In vitro, mDAPTA has been shown to inhibit the release of CXCR4- and CCR5-tropic HIV from CD8-depleted peripheral blood mononucleated cells (PBMCs) isolated from HIV-uninfected patients and HIV-infected patients with viral load less than 50 copies/mL.8
mDAPTA has also been studied for its antiviral effect on the JC virus in individuals with progressive multifocal leukoencephalopathy (PML).9
Half-life (T½): In a Phase I study involving participants with AIDS or AIDS-related complex (ARC), peptide T demonstrated biphasic plasma kinetics following intravenous (IV) or intranasal (IN) administration, with a first compartment half-life of 30 to 60 minutes and a second plasma clearance time of 4 to 6 hours.10
Metabolism/Elimination: In a Phase I study, participants with AIDS or ARC received peptide T via IV or IN administration. After administration, the drug could not be detected in urine.10
Resistance: In a small study of 11 HIV-infected participants receiving peptide T either alone or in combination with their current ART for up to 32 weeks, no obvious CCR5 to CXCR4 co-receptor shift occurred, and treatment-resistant viruses did not emerge.11
Study Identifier: Not available
Phase: Not available
Study Purpose: Study to evaluate the antiviral and immunological benefits of peptide T
Study Population: Long-term HIV-infected participants with stable viral loads. Participants were either treatment-naive or treatment-experienced.
Dosing: Peptide T 2 mg administered intranasally three times a day for up to 32 weeks, given either alone or in combination with current ART.11
Selected Study Results:
- Peptides article, 2003: Antiviral and immunological benefits in HIV patients receiving intranasal peptide T (DAPTA).
Study Identifier: NCT00000392
Study Purpose: Study to determine the ability of peptide T to treat HIV-associated cognitive impairment
Study Population: HIV-infected adults with cognitive impairment and not receiving ART within 4 weeks of study entry or receiving stable ART for at least 12 weeks prior to entry
Dosing: Peptide T 2 mg administered intranasally three times a day for 6 months versus similarly administered placebo.12,13
Note: During this study, participant cerebrospinal fluid (CSF) and peripheral blood samples were frozen and stored. At a later time, the stored CSF and blood samples were used to conduct a retrospective analysis on the ability of peptide T to reduce viral load in a subgroup of participants from the original trial sample.6,7
Selected Study Results:
- Arch Neurol article, 1998: Randomized Double-blind Placebo-Controlled Trial of Peptide T for HIV-Associated Cognitive Impairment
- J Neurovirol article, 2006: Cerebrospinal and peripheral human immunodeficiency virus type 1 load in a multisite, randomized, double-blind, placebo-controlled trial of D-Ala1-peptide T-amide for HIV-1-associated cognitive-motor impairment
Monomeric DAPTA (reformulation of peptide T)
Study Identifier: NCT00951743
Study Purpose: Study to evaluate the safety and toxicity of mDAPTA and ability of mDAPTA to eliminate treatment-resistant HIV in peripheral blood mononuclear cells (PBMCs)
Study Population: HIV-infected, treatment-experienced adults receiving ART for at least 6 months and with suppressed viral loads of less than 200 copies/mL for at least 3 months prior to study entry
Dosing: mDAPTA 0.01 mg administered intranasally twice daily for 24 weeks versus similarly administered placebo, each in combination with ART.14
Selected Study Results: Not available
In a small study investigating the antiviral and immune benefits of peptide T in 11 HIV-infected participants, peptide T was considered safe, with no drug-associated toxicities reported. No nasal pathologies were detected.11
In the Phase II study (NCT00000392) of peptide T in patients with HIV-associated cognitive impairment, researchers indicated no "clinically significant" toxic effects associated with peptide T use were noted. However, significant differences in the severity of instances of mood disturbance occurred in the peptide T group versus the placebo group (seven Grade 3 events occurred in the peptide T group versus one in the placebo group). Investigators indicated that this difference could be attributed to more severe mood disturbances at study entry in the peptide T arm participants than in the placebo arm participants. In addition, rash was significantly more severe in the peptide T group than in the placebo group. Participants receiving peptide T had a higher prevalence (borderline significant) of nasal congestion, proteinuria, and eosinophilia than participants receiving placebo.12,13
Drug interactions related to adaptavir use are currently unknown.
- United States National Library of Medicine. ChemIDplus Advanced. Available at: http://chem.sis.nlm.nih.gov/chemidplus/rn/106362-34-9. Last accessed on June 2, 2016.
- National Institute of Allergy and Infectious Diseases (NIAID). NIAID ChemDB, HIV Drugs in Development. Available at: http://chemdb.niaid.nih.gov/DrugDevelopmentHIV.aspx. Last accessed on June 2, 2016.
- RAPID Pharmaceuticals: Press Release, dated March 4, 2014. RAPID Acquires All Peptide T Clinical Data. Available at: http://www.rapidpharma.com/rapid-acquires-all-peptide-t-clinical-data/. Last accessed on June 17, 2015.
- RAPID Pharmaceuticals website. Monomeric DAPTA – the long path to discovery. Available at: http://www.rapidpharma.com/en/research-developement/technology-platform/path-to-discovery.html. Last accessed on March 31, 2014.
- Polianova MT, Ruscetti FW, Pert CB, Ruff MR. Chemokine receptor-5 (CCR5) is a receptor for the HIV entry inhibitor peptide T (DAPTA). Antiviral Res. 2005 Aug;67(2):83-92. Available at: http://www.researchgate.net/profile/Maria_Polianova/publication/8658079_Update_on_D-ala-peptide_T-amide_%28DAPTA%29_a_viral_entry_inhibitor_that_blocks_CCR5_chemokine_receptors/links/5436af3e0cf2643ab9881308.pdf. Last accessed on June 2, 2016.
- Pollicita M, Ruff MR, Pert CB, et al. Profound anti-HIV-1 activity of DAPTA in monocytes/macrophages and inhibition of CCR5-mediated apoptosis in neuronal cells. Antivir Chem Chemother. 2007;18(5):285-95. Available at: http://avc.sagepub.com/content/18/5/285.long. Last accessed on June 2, 2016.
- Goodkin K, Vitiello B, Lyman WD, et al. Cerebrospinal and peripheral human immunodeficiency virus type 1 load in a multisite, randomized, double-blind, placebo-controlled trial of D-Ala1-peptide T-amide for HIV-1-associated cognitive-motor impairment. J Neurovirol. 2006 Jun;12(3):178-89. Available at: http://www.researchgate.net/profile/Benedetto_Vitiello/publication/6909865_Cerebrospinal_and_peripheral_human_immunodeficiency_virus_type_1_load_in_a_multisite_randomized_double-blind_placebo-controlled_trial_of_D-Ala1-peptide_T-amide_for_HIV-1-associated_cognitive-motor_impairment/links/09e4150805c0ab6304000000.pdf. Last accessed on June 2, 2016.
- Agrawal L, Ducoudret O, Baichoo N, Laznicka M, Ruff M, Pert C. mDAPTA, a potent CCR5 receptor blocker, prevents viral recovery from CD8-depleted patient PBMCs with VL< 50 background. Abstract presented at: 18th International AIDS Conference; July 18-23, 2010; Vienna, Austria. Abstract TUPE0017. Available at: http://www.abstract-archive.org/. Last accessed on June 2, 2016.
- Rapid Pharmaceuticals. PML. Available at: http://www.rapidpharma.com/pml-rap-101/. Last accessed on June 2, 2016.
- Ruff MR, Smith C, Kingan T, et al. Pharmacokinetics of peptide T in patients with acquired immunodeficiency syndrome (AIDS). Prog Neuropsychopharmacol Biol Psychiatry. 1991;15(6):791-801. Available at: http://www.ncbi.nlm.nih.gov/pubmed/1763193. Last accessed on June 2, 2016.
- Polianova MT, Ruscetti FW, Pert CB, et al. Antiviral and immunological benefits in HIV patients receiving intranasal peptide T (DAPTA). Peptides. 2003 Jul;24(7):1093-8. Available at: http://www.rapidpharma.com/uploads/media/2003-02_Publication.pdf. Last accessed on March 31, 2014.
- Heseltine PN, Goodkin K, Atkinson JH, et al. Randomized Double-blind Placebo-Controlled Trial of Peptide T for HIV-Associated Cognitive Impairment. Arch Neurol. 1998 Jan;55(1):41-51. Available at: http://archneur.jamanetwork.com/article.aspx?articleid=773550. Last accessed on June 2, 2016.
- National Institute of Mental Health (NIMH). Phase II Study of the Efficacy of Peptide T in HIV-Positive Individuals With Cognitive Impairment. In: ClinicalTrials.gov. Bethesda (MD): National Library of Medicine (US). Registered on January 17, 2000. NLM Identifier: NCT00000392. Available at: https://www.clinicaltrials.gov/ct2/show/NCT00000392. Last accessed on June 2, 2016.
- Rapid Laboratories Inc. Safety and Efficacy of ADAPTAVIR's Ability to Eliminate Treatment-Resistant Infectious Virus in the Blood Cellular Reservoir (PBMCs) of HIV Patients With Suppressed Plasma Viral Load. In: ClinicalTrials.gov. Bethesda (MD): National Library of Medicine (US). Registered on July 31, 2009. NLM Identifier: NCT00951743. Available at: https://www.clinicaltrials.gov/ct2/show/NCT00951743. Last accessed on June 2, 2016.
Last Reviewed: June 15, 2016